DNA EXTRACTION

A rougher method of isolating DNA (and RNA) from an onion where its tissues were broken up mechanically and household detergent was used to degrade the cell and its membranes.
© c-lab 2004, onion
© c-lab 2004, onion
© c-lab 2004, add washing liquid to beaker
© c-lab 2004, add washing liquid to beaker
© c-lab 2004, add salt to washing up liquid
© c-lab 2004, add salt to washing up liquid
© c-lab 2004, dissolve with water
© c-lab 2004, dissolve with water
© c-lab 2004, stir well to dissolve the salt in the solution
© c-lab 2004, stir well to dissolve the salt in the solution
© c-lab 2004, chop the onion
© c-lab 2004, chop the onion
© c-lab 2004, place into salty washing up liquid place in water bath
© c-lab 2004, place into salty washing up liquid place in water bath
© c-lab 2004, cool mixture in ice bucket
© c-lab 2004, cool mixture in ice bucket
© c-lab 2004, blend for 4 seconds
© c-lab 2004, blend for 4 seconds
© c-lab 2004, pour blended mixture through filter
© c-lab 2004, pour blended mixture through filter
© c-lab 2004, this seperates cell wall material
© c-lab 2004, this seperates cell wall material
© c-lab 2004, add 3 drops of protease enzyme extract
© c-lab 2004, add 3 drops of protease enzyme extract
© c-lab 2004, mix
© c-lab 2004, mix
© c-lab 2004, add ice cold methylated spirits into mixture
© c-lab 2004, add ice cold methylated spirits into mixture
© c-lab 2004, onion DNA
© c-lab 2004, onion DNA
© c-lab 2004, place into microtube for storage in freezer
© c-lab 2004, place into microtube for storage in freezer