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  London Christmas Tree: Oh no! Contamination - but there is good news...

I entered the lab - half down with a hard-to-shake flu. I had not been to check on our Christmas Tree for days. I opened the blue drawer and began inspecting bottles, tubes and plates. The unknown plant seemed uneffected by hormone treatment. No roots, no contamination but a slight darkening (phenol?).

The unknown plant - no root

I inspected the plated. Oh no!!! Contamination! A white snow-covering fungi was powdering the christmas tree and thriving, creating a belt on the agar.

Christmas tree contaminated - by a snow looking fungi

Traces of fungi was also observed on the needle plate. Ok! These plates are goners...

Needles in a dish - contamination spreading!

But wait! One of the tube containing Auxin hormone showed tiny roots sticking out from the bottom of the wood!! 

Poking roots!

...and from a different angle...yeah...I think those are roots...

Looks like roots!

Very odd though - as the fluid colour had darkened. Only one of the tubes had this happen to it, the others containing needles and the unknown plant remained unchanged. 

The needle part did not produce roots...

Now we will have to be careful. We only have one! I will try to move it into media tomorrow...

 
 
Other References: Cloning London's Christmas Tree
London's Christmas Tree Clone: Day 2
C-LAB:blog: Cloning London's Christmas Tree
C-LAB:blog: London Christmas Tree: Oh no! Contamination - but there is good news...
 
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